1 edition of Uptake and distribution of C-tepa in boar spermatozoa after in vitro treatment. found in the catalog.
Uptake and distribution of C-tepa in boar spermatozoa after in vitro treatment.
by Agricultural Research (Northeastern Region), Science and Education Administration, U.S. Dept. of Agriculture, [Available from the Insect Reproduction Laboratory, Agricultural Environmental Quality Institute, Beltsville Agricultural Research Center] in [Beltsville, Md.]
|Series||Agricultural research results -- 12.|
|Contributions||United States. Science and Education Administration. Agricultural Research. Northeastern Region.|
|The Physical Object|
|Pagination||iv, 7 p. ;|
In the present study, the effects of lipofection and other augmentation techniques, such as sperm freezing and spermatozoa treatment with triton X and DMSO, on exogenous DNA uptake by sheep spermatozoa and motility of sperms with plasmid uptake were by: 3. The Benefit of Semen Cryostorage on Certain Sperm Function Parameters Khalid S. Al –Azzawi Following in Vitro Activation mixed with 30% (EY) on certain sperm function parameters of semen after 24 hr cryostorage. Semen analysis was done as after sperm activation in vitro of washed semen with 30% (EY) mixed with (MTS).
Calcium influx into equine and bovine spermatozoa during in vitro capacitation Anim. Reprod., v.1, n, p, Oct./Dec. 97 tract, the development of in vitro systems that promote capacitation and fertilization has made it possible to study more carefully the specific requirements for sperm capacitation and fertilizing ability. In vitro fertilization in inbred BALB/c mice I: isotonic osmolarity and increased calcium-enhanced sperm penetration through the zona pellucida and male pronuclear formation - Volume 16 Issue 3 - Seiji Kito, Yuki OhtaCited by: 5.
The zona pellucida-induced acrosome reaction has been shown to be the major indicator of sperm-fertilizing ability (Liu et al., ), and spermatozoa from men with defective zona pellucida-induced acrosome reaction have reduced or no ability to penetrate the zona pellucida and fertilize oocytes either in vivo or in vitro (Liu et al., , ).Cited by: AKG ability to amplify the PAF biosynthesis in a monocyte cell line, which is known to be produced by mammalian sperm and to be an important activator of sperm motility, was used as a starting point to evaluate the effect of in vitro treatment of boar spermatozoa with natural 1-O-alkylglycerols (10 mM) on: (1) boar sperm motility; (2 Cited by:
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Get this from a library. Uptake and distribution of ¹⁴C-tepa in boar spermatozoa after in vitro treatment. [United States. Science and Education Administration. see more details environment of spermatozoa after their in vitro treatment to induce capacitation. Sperm-rich fractions of the ejaculate were collected from 4 boars boars Subject Category: Organism Groups see more details of proven fertility.
The semen was treated for capacitation as follows: 10 ml of semen was centrifuged at g 4 : M. Sansegundo, C. Matás, N. Marín, A. González, S. Ruiz.
The present results confirm the decapacitating effect of SP and suggest capacitating actions of HA (dose-related) and CCM (freshly prepared) on boar spermatozoa in vitro.
The unclear effects of frozen-thawed CCM and a low dose of HA on penetration rates of boar spermatozoa call for further researches of Cited by: x g for 5 minutes for sperm recovery. The pellet was resuspended to sperm/ml in different media according to the experiment, as stated in each case.
Evaluation of the effect of heparin concentration on the physiology of boar spermatozoa Spermatozoa ( sperm/ml) were suspended in CM and separated in three 1 ml by: The in Vitro Effect of Taurine on Boar Spermatozoa Quality. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 66(1): – The aim of this in vitro study was to evaluate the effects of taurine (TAU) supplementation on boar spermatozoa motility, viability, acrosome integrity and morphology.
Eighteen boar semen samplesAuthor: Dušan Paál, František Strejček, Eva Tvrdá, Grzegorz Formicki, Sabine Klein, Detlef Rath, Peter Massa. On the sixth day of in vitro culture after injection of the sperm head and subsequent stimulation with an electrical pulse, the rates of blastocyst formation were not significantly different.
Human sperm penetration of zona-free hamster eggs after storage of the semen for 48 hours at 2 degrees C to 5 degrees C. Fertil Steril ; 39 (4): – Author: Natalie A.
Clark, Jason E. Swain. At present, artificial insemination with frozen‐thawed boar sperm is unlikely to become a widespread technique, although it will remain in use at genetic‐selection farms. The main advantage of boar sperm cryopreservation is the storage of genetic material (germplasm banks) from valuable sires beyond the lifespan of the specific by: In vitro fertilization rates were determined for cryopreserved spermatozoa from control boars and boars supplemented with selenium from organic or inorganic sources.
Percentages of embryos cleaved and becoming blastocysts were greatest (P) for boars fed ppm organic selenium.
Dietary selenium may improve fertility of cryopreserved boar. The boar reproductive system consists of six different structures (testes, epididymis, deferent ducts, urethra, accessory sex glands, and penis) and the main function is the production and the. BiolResARTICLES.
Effect of heparin on in vitro capacitation of boar sperm. DORA G DAPINO 1,2; PATRICIA E MARINI 2,3 and MARCELO O CABADA 2,4. 1 Cátedra de Fisiología.
Facultad de Ciencias Veterinarias. UNR 2 División Biología del Desarrollo (IBR-CONICET) y Área Biología. Facultad de Ciencias Bioquímicas y by: the sperm-rich fraction of boar semen was treated in vitro for 10 min with an equal vol of 1% soln of (14)c-labeled tepa. on average, % was taken up in the spermatozoa; 69% was associated with the heads & 31% with the tails & acrosomes.
By use of a specific culture medium, capacitation is also made to occur in vitro. During in vitro capacitation, spermatozoa show motility change, such as from “activation” to “hyperactivation.” Just after swim up in a specific culture medium, spermatozoa are activated (movies 1, 3, and 5).Cited by: Sperm signal transduction mechanisms of capacitation and acrosome reaction Figure 1.
Schematic representation of the main events occurring under conditions leading to capacitation in vitro. Changes in membrane permeability to several ions have been described, among these Ca 2+ and bicarbonate (HCO 3.
Effect of mg TEPA/ml Treatment of Spermatozoa and Insemination Dose on Stage of Embryo Developmenta No. of sperm insemi- Treatment No. of No. of Percentage of ova nated of sperm does per ova 1 rcovered as:a cells () treatment recovered cell cells >4 Mean SEMI 1 Control 8 88 41 18 2 2 57 18 * * * TEPA 8 95 73 6 23 7 4 4 5 Control 8 Cited by: 4.
This work was designed to study how this ability is affected by different sperm treatments routinely used for in vitro fertilization (IVF) assay.
In this study, boar sperm samples from epididymal or ejaculated origin were processed by three different methods: left unwashed (NW group), washed in Dulbecco's phosphate-buffered saline supplemented with % BSA (BSA group), and washed on a PercollCited by: SPERM TRANSPORT IN THE FEMALE REPRODUCTIVE TRACT OF LIVESTOCK AND POULTRY by IMTIAZ AHMED QURESHI B.
of the semen from the fallopian tubes of the mare 50 minutes after mating. The stallion sperm in vitro can travel at the rate of approximately U per second (Yamane and Ito, ).SPERM TRANSPORT IN THE FEMALE.
The study attempted to clarify the role of total antioxidant capacity of seminal plasma (SP-TAC) on boar sperm survival and fertility after artificial insemination (AI). SP-TAC differed (P Cited by: TheriogenologyBIOCHEMICAL CHANGES IN BULL SPERMATOZOA DURING CAPACITATION IN VITRO I.
White,' L. Belanger, S. Hough, J. Ellington2 and R. Foote Department of Animal Science Cornell University Ithaca, NY Received for publication: Ap Accepted: Novem ABSTRACT To evaluate the metabolic changes of bull spermatozoa Cited by: 3.
Enzyme activities and motility of boar spermatozoa during hour low-temperature storage BJVM, 16, No 4 cate sperm damage (Singh et al., ; Pesch et al, ). For example, lactate dehydrogenase isoenzyme X (LDH-X) is an enzyme, specific for the reproductive tissue and is included in metabolic pro-cesses, which provide energy for survival.
Mechanisms of Fertilization in Mammals. Authors; Authors and affiliations C. G., Cordle, C. T., and Metz, C. B.,Inhibition of fertilization in vitro by treatment of rabbit spermatozoa with univalent isoantibodies to rabbit Changes in antigenic site distribution on rabbit spermatozoa after incubation in “capacitating” media Cited by: J.
Jurkiewicz et al. incubation, the mixture was added to the samples of boar spermatozoa at a concen-tration of 1 × sperm in μl of extender and incubated at 37°C for 4–5 h. Evaluation of sperm motility Sperm motility was evaluated on a microscope slide heated to 38°C.in vivo after the spermatozoa have been present in the female tract for a period of time or in vitro under certain conditions and media.
This has been widely reviewed by several authors (). There are several structural and functional aspects of sperm membranes that may contribute to the.